Automatic Construction of Statistical Shape Models for Protein Spot Analysis in Electrophoresis Gels

M Rogers, J Graham and R P Tonge, "Automatic Construction of Statistical Shape Models for Protein Spot Analysis in Electrophoresis Gels", Proceedings of Medical Image Understanding and Analysis, 2003. PDF

Abstract

Proteomics research relies heavily on electrophoresis gels, which are complex images containing many protein ‘spots’. The identification and quantification of these spots is a bottleneck in the proteomics workflow. We describe a statistical model of protein spot appearance that is both general enough to represent unusual spots, and specific enough to introduce constraints on the interpretation of complex images. We propose a robust method of automatic model construction that is used to circumvent manual model construction which is subjective and time-consuming. We show that the statistical model of spot appearance is able to fit to image data more closely than the commonly used spot parameterisations which are based solely on Gaussian and diffusion formulations.